Designations:

SUP-B15

Depositors:

 SD Smith

Biosafety Level:

1

Shipped:

frozen

Medium & Serum:

See Propagation

Growth Properties:

suspension

Organism:

Homo sapiens

Morphology:

lymphoblast

Source:

Organ: bone marrow 
Disease: acute lymphoblastic leukemia 
Cell Type: B lymphoblast;

Cellular Products:

immunoglobulin (cytoplasmic)

Permits/Forms:

In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression:

CD1a -; CD2 -; CD3 -; CD4 -; CD5 -; CD8 -; CD10 +; CD13 +; CD38 +; CD71 +; HLA DR +

DNA Profile (STR):

Amelogenin: X,Y 
CSF1PO: 11,12 
D13S317: 8,14 
D16S539: 11,12 
D5S818: 12,13 
D7S820: 10,11 
THO1: 6,9.3 
TPOX: 8,9 
vWA: 15,17

Cytogenetic Analysis:

46, XY; the following markers are present: t(9;22)(q34;q11), t(4;14) (p11;q24), der(4)t(1;4) (p11;q33), t(9;22) (q34;q11), der(10)t(3;10) (q25;q26), add(16); Philadelphia chromosome is present.

Age:

8 years

Gender:

male

Ethnicity:

Caucasian

Comments:

This line was was derived from malignant cells collected from the bone marrow of an 8 year old child with Philadelphia chromosome positive B cell ALL. 
The cells express multiple B lineage markers, but do not express T cell markers. 
The cells are positive for the beta-2-microglobulin, Leu12, My7 (CD13), OKT9 (CD71), OKT10 (CD38) and CALLA (CD10) antigens. [23068 ] 
They are are negative for CB1, Leu 1 (CD5), Leu2 (CD8), Leu3 (CD4), Leu4 (CD3), Leu5 (CD2), Leu6 (CD1a), Leu9, Leu M1 (CD15), My9 (CD33), surface immunoglobulin (sIg -) and Epstein-Barr virus. [23068 ]

Propagation:

ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
Temperature: 37.0°C

Subculturing:

Protocol: Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 5 X 10 exp5 viable cells/ml and maintain between 5 X 10 exp5 and 2 X 10 exp6 cells/ml. 
Medium Renewal: 2 to 3 times per week

Preservation:

Freeze medium: Complete growth medium 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor temperature

Doubling Time:

18 hrs

Related Products:

Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020

References:

22924: Fainstein E, et al. A new fused transcript in Philadelphia chromosome positive acute lymphocytic leukaemia. Nature 330: 386-389, 1987. PubMed: 2825022
22958: Clark SS, et al. Expression of a distinctive BCR-ABL oncogene in Ph1-positive acute lymphocytic leukemia (ALL). Science 239: 775-777, 1988. PubMed: 3422516
23068: Naumovski L, et al. Philadelphia chromosome-positive acute lymphoblastic leukemia cell lines without classical breakpoint cluster region rearrangement. Cancer Res. 48: 2876-2879, 1988. PubMed: 3162827
23302: Rubin CM, et al. Heterogeneity of genomic fusion of BCR and ABL in Philadelphia chromosome-positive acute lymphoblastic leukemia. Proc. Natl. Acad. Sci. USA 85: 2795-2799, 1988. PubMed: 2833755
23305: Kawasaki ES, et al. Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro. Proc. Natl. Acad. Sci. USA 85: 5698-5702, 1988. PubMed: 3165197