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產(chǎn)品名稱：Invitrogen 18162-016 DNA聚合酶I
貨 號(hào)：18162-016
規(guī) 格：250U
分 類： DNA/RNA 聚合酶
品 牌：Invitrogen
國(guó)產(chǎn)/進(jìn)口：進(jìn) 口
儲(chǔ)存溫度：-20°C
特惠價(jià)格：致 電
庫(kù)存狀態(tài)：現(xiàn) 貨
銷售商：上海慧穎生物科技有限公司
慧穎生物專業(yè)供應(yīng)Invitrogen 18162-016 DNA聚合酶I，常備現(xiàn)貨，*。主營(yíng)Life Invitrogen產(chǎn)品，囊括TRlzol ?Reagent、TRIZOL LS Reagent 、B-27添加劑、凝膠填料、常規(guī)化學(xué)試劑、培養(yǎng)基及原料試劑、各類胎牛血清、馬血清等，如有需要，詳詢。
描述：
DNA Polymerase I/DNase I is an optimized mixture of both enzymes for efficient nick translation of DNA.
Application: Labeling DNA with either radiolabeled or biotinylated nucleotides.
Source: DNase I is purified from bovine pancreas; DNA Polymerase I from E. coli λ lysogen NM 964.
Performance and Quality Testing: Performance tested in nick translation reaction.
Unit Definition: One unit of DNA Polymerase I in the absence of DNase I incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using a template?primer.
Unit Reaction Conditions: 50 mM potassium phosphate (pH 7.5), 6.7 mM MgCl2 , 1 mM 2-mercaptoethanol, 80 μg/ml template?primer, 32 μM dTTP, 69 nM [3H]dTTP, and enzyme in 100 μl for 30 min. at 37°C.
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