B27細胞培養添加劑invitrogen/Gibco（貨號17504-044）說明書

描述

B-27® 添加劑是無血清添加劑，用于海馬神經元和其他中樞神經系統（CNS）神經元的生長和保持其短期或長期活性。B-27® 添加劑是50X 的液體，可以與Neurobasal® 培養基或Neurobasal®-A 培養基組合使用，用于神經細胞培養而不需要再添加飼養層細胞。

B-27® 添加劑適和應用于大多數神經細胞培養。對于特別的應用，我們提供 其他配方的B-27® 添加劑.

質量控制檢測 
B-27® 添加劑 進行了18天妊娠的原代大鼠（Sprague Dawley） 的胚胎海馬神經元生長檢測。 通過了細菌和真菌的檢測. B-27® 也做了1X 濃度的內毒素水平測試。 

僅供研究使用。除非另有說明，不得用于任何動物或人類治療或診斷。

詳細說明

B27細胞培養添加劑invitrogen/Gibco（貨號17504-044）說明書 常用規格

引文和參考文獻 （17）

• D2 receptors inhibit the secretory process downstream from calcium influx in dopaminergic neurons: implication of K+ channels.

作者

Congar P; Bergevin A; Trudeau LE

雜志

J Neurophysiol （2002） 87:1046-1102

產品用途

Using a culture system in which isolated DAergic neurons establish self-innervating synapses （"autapses"） that release both DA and glutamate, we studied the mechanism by which presynaptic D2 receptors inhibit glutamate-mediated excitatory postsynaptic currents （EPSCs）. 

ID:

PN64425

• Decreased synaptic vesicle recycling efficiency and cognitive deficits in amphiphysin 1 knockout mice.

作者

Di Paolo Gilbert; Sankaranarayanan Sethuraman; Wenk Markus R; Daniell Laurie; Perucco Ezio; Caldarone Barbara J; Flavell Richard; Picciotto Marina R; Ryan Timothy A; Cremona Ottavio; De Camilli Pietro;

雜志

Neuron （2002） 33:789-804

產品用途

Primary cultures of cortical neurons were prepared. Cells were maintained up to 3 weeks in Neurobasal/B27 medium at 37°C in a 5% CO2 humidified incubator. 

ID:

PN47418

• Blocking HES1 expression initiates GABAergic differentiation and induces the expression of p21（CIP1/WAF1） in human neural stem cells.

作者

Kabos Peter; Kabosova Andrea; Neuman Toomas;

雜志

J Biol Chem （2002） 277:8763-8769

產品用途

Resected human fetal brain tissue was placed into ice cold DMEM/F-12 with penicillin/streptomycin for further dissection. The tissue was cut into small pieces and trypsinized （0.02 mg/ml trypsin in versene （Invitrogen） at 37 °C for 10 min. Cell suspension was centrifuged at 400 rpm for 5 min, the pellet was washed once with DMEM/F-12 and plated at a density of 5000-10,000 viable cells/ml in the growth medium DMEM/F-12, B27 supplement （Invitrogen）. Cells were washed with PBS （Invitrogen）  

ID:

PN63471

• Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death.

作者

Lesuisse Christian; Martin Lee J;

雜志

J Neurobiol （2002） 51:9-23

產品用途

A long-term cell culture system was used to study maturation, aging, and death of cortical neurons. 

ID:

PN60461

• An improved method for the expression of TRH in serum-supplemented primary cultures of fetal hypothalamic cells.

作者

Joseph-Bravo Patricia; Pérez-Martínez Leonor; Lezama Leticia; Morales-Chapa Claudia; Charli Jean Louis;

雜志

Brain Res Brain Res Protoc （2002） 9:93-104

產品用途

Primary cultures of dispersed cells from fetal nervous tissue are extensively used for studying multiple neuronal properties 

ID:

PN62028

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慧穎生物主營：生化試劑、抗體、重組蛋白、細胞生物、分子生物、蛋白研究、微生物、Elisa試劑盒、耗材、標準品、 標準溶液……

abcam、ABI、Axygen、corning、CST、Gibco、Hyclone、invitrogen、merck、Millipore、Qiagen、R&D、Roche、santa、sigma、Thermo等進口品牌……

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